Table 1.
Specific activity of the 3AP dehydrogenases detected from high-throughput enzymatic screening
| Gene ID | Gene name | Predicted function | Specific activity (μM min−1 μg−1) |
|---|---|---|---|
| ACIAD0960 | gabD like | Putative succinate-semialdehyde dehydrogenase [NADP+] (GabD-like) | 5.47 ± 0.08a |
| ACIAD1009 | betB | NAD+-dependent betaine aldehyde dehydrogenase | 1.80 ± 0.02b |
| ACIAD2018 | acoD | Acetaldehyde dehydrogenase II (ACDH-II) | 5.71 ± 0.06b |
| ACIAD3339 | adhA | Alcohol dehydrogenase | 1.99 ± 0.03b |
Activities reported represent the greater value obtained with either 2 mM NADP+ or NAD+.
a
Value obtained with NADP+.
b
Value obtained with NAD+. Values are ±SD from two independent experiments conducted in 100 μl 50 mM Tris–HCl, pH 9.0, 200 mM NaCl, and 15% glycerol containing 4 mM 3AP.