Table 2.

Kinetic parameters for the dehydrogenases of the alternative route for β-alanine synthesis

Enzyme ID	Enzyme name	Substrate	kcat (s−1)	Km (μM)	kcat/Km (s−1 M−1)
ACIAD0960	GabD-like	3APa	3.86 ± 0.40	2934 ± 306	1.3 103
		NADP+b		3718 ± 1138	1.0 103
		NAD+b	1.68 ± 0.04	4507 ± 363	3.7 102
ACIAD1009	BetB	3APc	1.48 ± 0.16	82 ± 17	1.8 104
		NAD+d		20 ± 4	7.4 104
		NADP+d	2.43 ± 0.12	339 ± 67	7.2 103
ACIAD2018	AcoD	3APe	1.55 ± 0.35	3559 ± 755	4.4 102
		NAD+f		124 ± 49	1.3 104
ACIAD3339	AdhA	3APg	0.93 ± 0.07	141 ± 28	6.6 102
		NAD+h		1803 ± 305	5.2 102

Values are the average of two replicates.

^aNADP⁺ concentration was 16 mM.

^b3AP concentration was 15 mM.

^cNAD⁺ concentration was 2 mM.

^d3AP concentration was 2.5 mM.

^eNAD⁺ concentration was 2 mM.

^f3AP concentration was 20 mM.

^gNAD⁺ concentration was 9 mM.

^h3AP concentration was 2 mM.