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. 2022 May 24;298(7):102067. doi: 10.1016/j.jbc.2022.102067

Table 4.

Kinetic properties of Ddc

Enzyme ID Enzyme name Reaction Substrate Km (μM) kcat (s−1) kcat/Km (s−1 M−1)
ACIAD1211 Ddc Decarboxylase DAB 1585 ± 257 12.1 ± 1.1 7.6 103
Amine oxidase DAP 710 ± 113 0.009 ± 3 10−4 13

Amine oxidase activity was determined in a spectrophotometric coupled assay by measuring 3AP oxidation in the presence of BetB and 1 mM NAD+, whereas decarboxylase activity was determined by LC/MS/MS, as described under the Experimental procedures section. Values are the average of three replicates.