FIG. 1.

Partial restriction map of the plasmid pB8 and deletion analysis. Boxes indicate the insert DNA fragments. 5-Oxoprolinase activities were assayed with sonic extracts prepared from the transformants containing various plasmids. The enzyme activities of each transformant are expressed as values relative to those of E. coli DH5α harboring pB8 at 25°C. The large arrow indicates the position and direction of the enzyme gene. Small arrows show orientations of the lac promoter relative to the insert DNA.