TABLE 1.

Purification of the recombinant N-38A enzyme from E. coli JM109 harboring pKK/N-38A cells

Purification step	Total protein (mg)	Total activity (mU)	Specific activity (mU/mg)	Purification (fold)	Yield (%)
Cell extract	33,100	619,000	18.7	1	100
Butyl-Toyopearl 650M	1,000	263,000	263	14	42.5
Butyl-Toyopearl 650M	114	221,000	1,940	104	35.7
DEAE-Sepharose fast flow	60.4	152,000	2,520	135	24.6