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Effect of various chemical reagents and metal ions on N-38A enzyme activity
| Addition (1 mM) | Inhibition (%)
|
|
|---|---|---|
| Recombinant | Authentic | |
| pCMB | 92.5 | 89.6 |
| Iodoacetamide | 10.7 | 10.3 |
| N-Ethylmaleimide | 25.4 | 23.8 |
| EDTA | 80.6 | 85.9 |
| o-Phenanthroline | 85.3 | 82.8 |
| KCN | 0 | 0 |
| NaN3 | 0 | 0 |
| SDS | 43.9 | 50.6 |
| HgCl2 | 92.6 | 90.9 |
| FeCl2 | 12.2 | 10.2 |
| FeCl3 | 8.5 | 8.6 |
| CoSO4 | 36.9 | 38.9 |
| ZnCl2 | 0 | 0 |
| MnCl2 | 10.9 | 9.5 |
| CaCl2 | 2.1 | 2.9 |
| MgSO4 | 35.6 | 38.9 |
| CuSO4 | 90.5 | 92.1 |
