. 2022 Jun 21;12:10394. doi: 10.1038/s41598-022-14545-8

Table 1.

Summary of predicted physiochemical properties of peptides^24,25.

Peptide	Sequence	MW*²⁶	pI**	Negatively charged^aa	Positively charged^aa	Instability index^#	Aliphatic index^##	Grand average of hydrophobicity (GRAVY)^{^}	Antigenicity (threshold 0.4)^{^^}
A1	SSSDLSDSSISNDYLVANSG	2018	3.42	3	0	48.02	78	− 0.375	0.7241
A2	SNDYLVANSGDDSVASSSAS	1946	3.42	3	0	38.39	63.5	− 0.36	1.0194
A3	DDSVASSSASSSIAADDSDL	1900	3.24	5	0	63.04	73.5	− 0.21	1.0257
A4	SSIAADDSDLSNNASSSNVN	1968	3.42	3	0	43.78	68.5	− 0.61	0.999
A5	SNNASSSNVNFENEVLSTNN	2141	3.79	2	0	13.73	53.5	− 0.97	0.9475
A6	FENEVLSTNNNEDTESEIVK	2311	3.83	6	1	36.94	68	− 1.12	0.7911
A7	NEDTESEIVKDSKNQLSSSS	2197	4.18	5	2	79.2	53.5	− 1.44	0.739
A8	DSKNQLSSSSLQASTKTKTT	2112	9.7	1	3	60.55	44	− 1.195	1.1344
A9	LQASTKTKTTLKGSGSSVYR	2113	10.46	0	4	14.71	58.5	− 0.71	1.2187
A10	LKGSGSSVYRGNPYYVTLTD	2177	8.43	1	2	− 9.59	68	− 0.495	1.0281
A11	GNPYYVTLTDSNGKVLASQK	2155	8.43	1	2	9.00	73	− 0.60	0.7261
A12	SNGKVLASQKVTFNILGKNY	2182	10	0	3	28.16	92.5	− 0.25	0.4959
A13	VTFNILGKNYTRTTDSKGVA	2185	9.7	1	3	39.92	73	− 0.36	1.3222
A14	TRTTDSKGVASININLAKGK	2074	10.29	1	4	28.37	83	− 0.53	1.5786
A15	SININLAKGKYNIACLYAGT	2127	9.1	0	2	84.14	112.5	0.29	0.8793
A16	YNIACLYAGTENYASSKLSV	2167	5.99	1	1	97.52	88	0.12	0.7904
A17	ENYASSKLSVALTVNLMSTK	2156	8.59	1	2	48.69	97.5	0.055	0.8185

*MW (molecular weight in g/mol).

**pI (isoelectric point).

^#Instability index (value of instability index smaller than 40 is predicted as stable, a value above 40 predicts that the peptide may be unstable).

^##Aliphatic index (larger values represent increase of thermostability of the peptide).

^{^}GRAVY (positive GRAVY values indicate hydrophobic nature of peptide and negative values indicate hydrophilic nature).

^{^^}Antigenicity index (values represent peptide antigenicity).