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. 2022 Mar 25;16(7):1730–1739. doi: 10.1038/s41396-022-01221-y

Fig. 6. Environmental and clinical isolates of P. aeruginosa promote dual biofilm formation.

Fig. 6

Ss was co-cultured with A environmental, B non-CF acute, or C CF non-mucoid and mucoid isolates of P. aeruginosa in TSBYE with 1% sucrose in a 96-well 16-h biofilm model. Biofilms were stained with crystal violet to measure biofilm biomass (n = 3 biological, 3 technical). Error bars indicate mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A + B). One-way ANOVA with Holm-Šίdák’s multiple comparisons test (C). **p < 0.01, ***p < 0.001, ****p < 0.0001.