Table 1.
Bacterial strains and plasmids.
| Strain | Characteristics | Reference/source |
|---|---|---|
| K12 (S. salivarius) | Wildtype | [51] |
| FRD1 (P. aeruginosa) | CF isolate, mucoid | [52] |
| PAO1 (P. aeruginosa) | Wound isolate, non-mucoid | [53] |
| PAO1ΔpslA (P. aeruginosa) | In-frame deletion of pslA | [27] |
| PAO1 pslA+ (P. aeruginosa) | Complemented PAO1ΔpslA with pslA gene | This study |
| PAO1ΔmucA (P. aeruginosa) | Deletion of mucA (PDO300) | [28] |
| PA14 (P. aeruginosa) | Wildtype | [29] |
| PAO1-GFP | PAO1 with carbR GFP plasmid | This study |
| Environmental isolates (P. aeruginosa) | E1-E10 non-mucoid, water isolate | This study |
| AC1 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC2 (P. aeruginosa) | Non-mucoid, wound isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC3 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC4 (P. aeruginosa) | Non-mucoid, bronchial wash isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC5 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC6 (P. aeruginosa) | Non-mucoid, blood isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC7 (P. aeruginosa) | Non-mucoid, bronchoalveolar lavage isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC8 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC9 (P. aeruginosa) | Non-mucoid |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC10 (P. aeruginosa) | Non-mucoid, tracheal aspiration isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC11 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC12 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC13 (P. aeruginosa) | Non-mucoid, urine isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC14 (P. aeruginosa) | Non-mucoid, nasal isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| AC15 (P. aeruginosa) | Non-mucoid, maxillary sinus isolate |
Dr. Bill Benjamin UAB Clinical Microbiology Lab |
| CF clinical isolates (P. aeruginosa) | CF1-CF3 non-mucoid, CF4-CF6 mucoid |
Dr. Susan Birket UAB CF Center |
| DH10b (E. coli) | Host strain for cloning | Thermo Fisher |
| pBKSNS1 | pBluescript K(+) ligated to pslA gene | This study |