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. 2022 Jun 21;12:10419. doi: 10.1038/s41598-022-14183-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expansion is limited to PM alleles on the active X chromosome. PCR profiles of two PM female carriers without (top panels) and with (bottom panels) HpaII digestion show the loss of the larger of the two allele populations on predigestion of the template with HpaII which preferentially eliminates alleles on the active X chromosome.