Table 2.

Growth of recovered C. albicans isolates in ASM with fluconazole.

Time (h)	MIC (µg mL−1) of fluconazole against isolates collected from mono-species culture challenged with:
	2× MIC fluconazole				5× MIC fluconazole
3	2.5	2.5	2.5	10	2.5	2.5	2.5	2.5
8	5	10	10	10	5	5	10	10
24	5	5	10	10	2.5	5	5	10
48	2.5	2.5	2.5	2.5	1	2.5	2.5	2.5
	MIC (µg mL−1) of fluconazole against isolates collected from polymicrobial co-culture challenged with:
	2× MIC fluconazole				5× MIC fluconazole
3	5	10	10	10	5	5	5	10
8	5	10	10	10	5	5	10	10
24	5	5	10	10	2.5	5	5	10
48	2.5	5	10	10	1	1	1	2.5

Number of C. albicans SC3541 isolates able to grow in ASM containing the indicated concentration of fluconazole (in µg mL⁻¹). Four isolates were collected at each time point (3 h, 8 h, 24 h and 48 h) after challenge of each culture with 2× or 5× MIC fluconazole (i.e., 4 × 4 × 2 = 32 isolates in all). Isolates were randomly selected from agar spreads of appropriately diluted single-species (upper panel) and PA/SA/CA mixed-species (lower panel) steady-state continuous-flow cultures. The ability of each isolate to grow in different concentrations of fluconazole was then determined. The results were confirmed over three independent experiments using fresh batches of ASM. Shades of grey indicate resistance to colistin relative to WT; dark grey < light grey < white.