. 2022 Mar 19;16(7):1694–1704. doi: 10.1038/s41396-022-01218-7

Table 3.

Non-synonymous SNPs and indels associated with the colistin resistant P. aeruginosa isolates.

Isolate	Culture type	Time (h)	Locus	Gene	Product	Type	Effect	NT position	AA position
A	Mono	1	PA3154	wzy	B-band O-antigen polymerase	snp	missense	1238/1317	413/438
			PA5044	pilM	Type 4 fimbrial biogenesis protein	ins	frameshift	531/1065	177/357
			PA4496	dppA1	ABC transporter	ins	intragenic
B	Mono	1	PA3159	wbpA	UDP-N-acetyl-D-glucosamine 6-dehydrogenase	snp	missense	150/1311	50/436
			PA5044	pilM	Type 4 fimbrial biogenesis protein	ins	frameshift	531/1065	177/357
			PA4496	dppA1	ABC transporter	ins	intragenic
C	Poly	1	PA3155	wbpE	UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase	snp	missense	701/1080	234/359
			PA5044	pilM	Type 4 fimbrial biogenesis protein	ins	frameshift	531/1065	177/357
			PA4496	dppA1	ABC transporter	ins	intragenic
			PA5001	ssg	Hypothetical protein	snp	missense	569/957	190/318
D	Poly	1	PA3154	wzy	B-band O-antigen polymerase	ins	frameshift	129/1317	43/438
D	Poly	1	PA4496	dppA1	ABC transporter	ins	intragenic
E	Mono	8	PA3154	wzy	B-band O-antigen polymerase	ins	frameshift	129/131	43/438
			PA3805	pilF	Type 4 fimbrial biogenesis protein	snp	missense	581/759	194/252
			PA4496	dppA1	ABC transporter	ins	intragenic
F	Mono	8	PA3154	wzy	B-band O-antigen polymerase	snp	stop gained	266/1317	89/438
			PA4552	pilW	Type 4 fimbrial biogenesis protein	del	frameshift	561/825	187/274
			PA4496	dppA1	ABC transporter	ins	intragenic
G	Poly	8	PA4552	pilW	Type 4 fimbrial biogenesis protein	del	frameshift	561/825	187/274
G	Poly	8	PA4496	dppA1	ABC transporter	ins	intragenic
H	Poly	8	PA3154	wzy	B-band O-antigen polymerase	ins	frameshift	266/1317	89/438
H	Poly	8	PA4496	dppA1	ABC transporter	ins	intragenic

Summary of non-synonymous SNPs and indels identified in the genome of PA isolates resistant to the action of 1 mg mL⁻¹ colistin when compared with the re-sequenced reference genome of the progenitor (PAO1). All isolates sequenced were collected from a steady-state continuous-flow culture after 1 h or 8 h exposure (as indicated) to 8 µg mL⁻¹ colistin (2× MIC). Culture type identifies whether the isolate was obtained from a mono-species (Mono) or polymicrobial (Poly) culture. Mutation type is abbreviated to ins (insertion), del (deletion) or snp (single nucleotide polymorphism). NT position and AA position denote the location of the nucleotide or amino acid change in the gene or encoded protein, respectively.