Fig. 3. SPARCED model recapitulates experimental observations and deterministic/hybrid (deterministic + stochastic) simulation results of the Bouhaddou2018 model.

a Summary of comparisons of SPARCED model deterministic simulations to Bouhaddou2018 model simulations. The area under the curve (AUC) values of each simulation (see Supplementary Fig. 12) are calculated and plotted for the two model results. b Simulation results from Bouhaddou2018 model (line) and SPARCED-nf model (triangle) run on Kubernetes cluster workflow are the same as SPARCED model (circle) results. Comparisons of selected panels from (a) are shown only. c Experimental and stochastic simulation results from Bouhaddou2018 model are reproduced by SPARCED model simulations. Each dot is a different condition, explained in Supplementary Fig. 13a. Error bars show experimental or simulation standard error of the mean. Simulations are of at least 100 cells, and three independent experimental observations where applicable. d Stochastic simulation of 100 cells recapture protein level trajectories (active p53, Cyclin A, and cPARP) from older model qualitatively. Panels with blue background are SPARCED simulations and white background panels are from Bouhaddou2018 model. 100 stochastic cells are stimulated with EGF + Insulin for 72 h before Etoposide treatment for another 72 h. Etoposide is stimulated also with EGF + Insulin. Results for Etoposide treatment without prior growth factor stimulations are shown in Supplementary Fig. 13b. e Quantification of results in (d) shows that SPARCED model simulations coincide with earlier observations in percentage of death induced by etoposide treatment. See Supplementary Fig. 13c for the effect of no growth factor stimulation before Etoposide treatment. Gray bars represent mean ± s.e.m. of three independent biological replicates (dots). Blue and orange bars represent the percentage of dead cells at specified time ± s.e.m. Source data are provided in Source Data.