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Comparison of detection sensitivities of the sequence capture-PCR (A), gel filtration-PCR (B), and IMS-PCR (C) methods as measured by identification of IS2404 DNA in environmental samples spiked with M. ulcerans. Lane 1, 104 cells; lane 2, 103 cells; lane 3, 102 cells; lane 4, 10 cells; lane 5, 1 cell; lane 6, no cells; lane 7, PCR positive control; lane 8, PCR negative control; lane M, 100-bp ladder size marker.
