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. 2000 Aug;66(8):3206–3213. doi: 10.1128/aem.66.8.3206-3213.2000

FIG. 3.

FIG. 3

Identification of M. ulcerans from the Langwarrin swamp (Fig. 2, site B) in the Frankston-Langwarrin region based on detection of the 492-bp IS2404 PCR product (A and B) and the 332-bp IS2606 PCR product (C and D). The PCR products were separated by gel electrophoresis (A and C) and were detected by Southern hybridization (B and D). The total mycobacteria in the samples were identified by detection of the 1,030-bp mycobacterial 16S rDNA PCR product and the 331-bp IPC PCR product (E). Lane 1, water from site B, area 2; lane 2, sediment from site B, area 2; lane 3, plant sample (Triglochin spp.) from site B, area 2; lane 4, water from site B, area 1; lane 5, sediment from site B, area 1; lane 6, detritus from site B, area 1; lane 7, sterile water procedural control; lane 8, PCR positive control; lane 9, PCR negative control; lanes M and M1, molecular weight markers (100-bp ladder [Gibco, BRL] and PGEM size ladder [Promega], respectively).