Maximum-parsimony tree based on partial rRNA 16S sequences from environmental mycobacterial isolates detected by IMS in IS2404-positive samples, closely related species identified by BLASTN analysis, and other mycobacterial species of clinical significance. The designations for the mycobacteria isolated by the IMS method begin with the prefix JKD, which is followed by a unique four-digit number. The region from which each isolate was cultured is indicated in parentheses, as follows: PI, Phillip Island; FLW, Frankston-Langwarrin. The tree was rooted by using N. asteroides as an outgroup.