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. 2000 Aug;66(8):3206–3213. doi: 10.1128/aem.66.8.3206-3213.2000

TABLE 4.

MPN estimates for the concentrations of M. ulcerans cells in IS2404 PCR-positive samples collected from the Frankston-Langwarrin and Phillip Island regions

Site Collection date (day/mo/yr) Sample type Amt of original sample analyzed per replicatea Results
No. of positive tubes per dilution MPN per 100 ml or 100 g (wet wt) of sampleb
Langwarrin swamp, site B1 28/1/98 Water 150 ml (1) 1 0.80 (0.19–3.37)
75 ml (2) 1
10/2/98 Water 75 ml (3) 1 0.43 (0.06–3.07)
28/1/98 Detritus 1.5 g (4) 2 38 (10–154)
3/2/98 Detritus 1.5 g (2) 1 30 (4–210)
Phillip Island Golf Club
 Tank 3/3/98 Water 150 ml (2) 1 0.30 (0.04–2.13)
 Pump shed 3/3/98 Water 150 ml (3) 1 0.21 (0.03–1.54)
 Irrigation system 24/3/98 Water 300 ml (4) 3 0.50 (0.20–1.25)
75 ml (4) 2
 Dam 16/6/98 Water 75 ml (3) 2 1.0 (0.25–4.22)
 Dam 3/3/98 Detritus 1.5 g (3) 2 51 (12–211)
a

The numbers in parentheses are numbers of replicates. 

b

The numbers in parentheses are 95% confidence intervals.