Table 1.
Metagenome-assembled genomes (MAGs) from EA (upper Table A) and TSB (lower Table B) cultures in the +As condition.
| A | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| MAG | Bin | Marker lineagea | Completeness (%) | Contamination (%) | Strain heterogeneity (%) | Genome size (Mbp) | Community (%) | GC content | ArsM-encoding genes | Binnerb |
| 1 | 36 | Bacteroidales (o) | 98.5 | 0.4 | 0.0 | 3.8 | 7.26 ± 0.32 | 39.1 | 0 | A |
| 2 | 15 | Clostridiales (o) | 98.7 | 0.0 | 0.0 | 2.2 | 0.52 ± 0.06 | 58.4 | 0 | B |
| 3 | 21 | Clostridiales (o) | 95.2 | 0.0 | 0.0 | 4.3 | 5.73 ± 0.24 | 28.5 | 0 | C |
| 4 | 4 | Clostridiales (o) | 90.7 | 0.0 | 0.0 | 2.1 | 0.30 ± 0.03 | 57.5 | 0 | A |
| 5 | 24 | Clostridiales (o) | 97.8 | 0.3 | 0.0 | 2.0 | 25.21 ± 0.23 | 43.2 | 0 | A |
| 6 | 9 | Clostridiales (o) | 100.0 | 1.3 | 50.0 | 3.2 | 12.68 ± 0.68 | 54.9 | 0 | A |
| 7 | 35 | Clostridiales (o) | 98.0 | 3.3 | 55.6 | 5.3 | 0.84 ± 0.21 | 44.0 | 0 | A |
| 8 | 31 | Clostridiales (o) | 97.9 | 3.5 | 0.0 | 3.8 | 1.48 ± 0.12 | 28.2 | 1 | A |
| 9 | 20 | Clostridium (g) | 97.2 | 2.2 | 0.0 | 3.4 | 0.70 ± 0.06 | 30.1 | 1 | C |
| 10 | 18 | Clostridium (g) | 96.5 | 2.9 | 16.7 | 4.0 | 0.27 ± 0.05 | 30.0 | 0 | A |
| 11 | 11 | Deltaproteobacteria (o) | 99.2 | 0.7 | 100.0 | 3.4 | 0.63 ± 0.05 | 57.4 | 2 | BC |
| 12 | 33 | Deltaproteobacteria (o) | 100.0 | 1.2 | 0.0 | 3.3 | 12.71 ± 0.49 | 57.8 | 3 | BC |
| 13 | 28 | Firmicutes (p) | 99.9 | 0.0 | 0.0 | 2.5 | 1.02 ± 0.10 | 47.2 | 0 | B |
| 14 | 27 | Firmicutes (p) | 91.9 | 3.3 | 92.3 | 3.1 | 0.84 ± 0.07 | 49.2 | 1 | BC |
| 15 | 8 | Lactobacillales (o) | 99.6 | 0.0 | 0.0 | 2.7 | 3.30 ± 0.61 | 36.8 | 0 | A |
| 16 | 1 | Lactobacillales (o) | 99.3 | 4.6 | 0.0 | 4.1 | 0.99 ± 0.05 | 39.1 | 0 | BC |
| 17 | 16 | Selenomonadales (o) | 100.0 | 1.5 | 0.0 | 2.2 | 2.60 ± 0.24 | 41.3 | 0 | C |
| B | ||||||||||
| MAG | Bin | Marker lineagea | Completeness (%) | Contamination (%) | Strain heterogeneity (%) | Genome size (Mbp) | Community (%) | GC content | N. of ArsM-encoding genes | Binnerb |
| 1 | 12 | Clostridiales (o) | 100.0 | 0.0 | 0.0 | 3.1 | 0.41 ± 0.03 | 54.8 | 0 | B |
| 2 | 9 | Clostridiales (o) | 98.9 | 0.0 | 0.0 | 4.7 | 1.26 ± 0.24 | 28.4 | 0 | C |
| 3 | 39 | Clostridiales (o) | 98.0 | 0.3 | 0.0 | 2.0 | 2.36 ± 0.51 | 43.2 | 0 | A |
| 4 | 4 | Clostridiales (o) | 99.3 | 0.7 | 100.0 | 2.7 | 4.77 ± 3.14 | 56.1 | 0 | A |
| 5 | 16 | Clostridiales (o) | 98.7 | 0.9 | 0.0 | 2.8 | 0.35 ± 0.21 | 35.7 | 0 | A |
| 6 | 19 | Clostridiales (o) | 99.2 | 1.1 | 0.0 | 3.5 | 0.39 ± 0.28 | 31.2 | 1 | BC |
| 7 | 1 | Clostridiales (o) | 98.7 | 1.3 | 50.0 | 2.6 | 0.25 ± 0.07 | 56.1 | 1 | A |
| 8 | 15 | Clostridiales (o) | 97.3 | 2.5 | 16.7 | 2.7 | 0.17 ± 0.02 | 60.5 | 0 | C |
| 9 | 28 | Clostridium (g) | 99.3 | 5.5 | 23.1 | 5.6 | 1.42 ± 0.30 | 30.1 | 2 | A |
| 10 | 27 | Clostridium (g) | 98.6 | 6.9 | 0.0 | 4.6 | 2.10 ± 0.60 | 32.3 | 1 | A |
| 11 | 32 | Deltaproteobacteria (o) | 94.8 | 0.0 | 0.0 | 3.1 | 0.21 ± 0.06 | 59.3 | 0 | BC |
| 12 | 38 | Deltaproteobacteria (o) | 98.3 | 1.8 | 50.0 | 3.4 | 0.81 ± 0.08 | 57.6 | 2 | BC |
| 13 | 10 | Enterobacteriaceae (f) | 96.6 | 0.7 | 33.3 | 4.3 | 0.39 ± 0.08 | 52.8 | 0 | B |
| 14 | 42 | Enterobacteriaceae (f) | 95.7 | 2.1 | 12.5 | 5.1 | 6.77 ± 0.35 | 56.3 | 0 | BC |
| 15 | 31 | Firmicutes (p) | 99.9 | 0.0 | 0.0 | 2.4 | 0.37 ± 0.06 | 47.6 | 0 | A |
| 16 | 33 | Firmicutes (p) | 100.0 | 0.6 | 0.0 | 3.2 | 1.97 ± 1.09 | 49.1 | 1 | BC |
| 17 | 7 | Lactobacillales (o) | 99.6 | 0.0 | 0.0 | 2.9 | 2.64 ± 0.52 | 36.5 | 0 | C |
| 18 | 5 | Lactobacillales (o) | 98.9 | 4.2 | 0.0 | 4.1 | 1.94 ± 0.81 | 39.1 | 0 | AB |
| 19 | 36 | Selenomonadales (o) | 100.0 | 1.5 | 0.0 | 2.3 | 0.81 ± 0.11 | 41.1 | 0 | A |
Marker lineage: taxonomic rank set by CheckM. Completeness and contamination (%): estimated completeness and contamination of genome as determined by CheckM from the presence/absence of single-copy marker genes and the expected colocalization of these genes. Strain heterogeneity: index between 0 and 100 where a value of 0 means no strain heterogeneity, high values suggest the majority of reported contamination is from closely related organisms (i.e., potentially the same species) and low values suggest the majority of contamination is from phylogenetically diverse sources. Proportion of binned proteins assigned to MAG (%): number of protein-coding genes assigned to the MAG divided by the total number of protein-coding genes binned. Community (%): sum of the number of reads mapped to the contigs in each MAG divided by the total number of reads mapped to all contigs including the unbinned contigs, and normalised to MAG size, assuming an average genome size for all unbinned populations.
a(p) phylum, (o) order, or (g) genus.
bA,B and C refer to MetaBAT 2, MaxBin 2.0 and CONCOCT respectively.