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. 2022 Jun 21;12:10479. doi: 10.1038/s41598-022-14326-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zones of gelatinization identified with H&E histology for healthy tissue (a), and 5s (c), 15s (e), and 60s (g) burns, and corresponding PLM data (b, d, f, h). Dashed lines in black (a, c, e, g) and red (b, d, f, h) show automatically-extracted surface and injury boundaries. Summary of results for Datasets 1 (i) and 2 (j) show good agreement between H&E and PLM data. Error bars represent standard deviation across N = 6 histological cross sections for each Dataset. Scale bars = 1 mm.