Fig. 5. Kir2.1-mediated Vm drives nutrient uptake by retaining nutrient transporters on the macrophage cell surface.
a–c Overlap analysis of differentially expressed genes of mouse BMDMs treated with 500 ng/ml LPS in the presence or absence of ML133 (25 μM), elevated [K+]e (50 mM), or gramicidin (1.25 μM) for 6 h (a), and pathway enrichment analysis of overlapped downregulated genes (b) and upregulated genes (c). LM: LPS + ML133; LG: LPS + Gramicidin; LK: LPS + KCl. d, e Western Blot analysis of autophagy (d) and AMPK activation (e) in mouse peritoneal macrophages treated with LPS in the presence or absence of ML133 (25 μM), elevated [K+]e (50 mM), or gramicidin (1.25 μM) for 6 h. f Membrane GLUT1 and 4F2hc expression FACS assays of peritoneal macrophages treated with or without 500 ng/ml LPS in the presence or absence of different doses of ML133 for 2 h (n = 3, mean ± SD). g, h Membrane GLUT1 and 4F2hc expression FACS assays of peritoneal macrophages treated with or without 500 ng/ml LPS in the presence or absence of elevated [K+]e (50 mM) or gramicidin (1 μM or 2 μM) for 2 h. (n = 3, mean ± SD). i Schematic of plasma membrane protein biotinylation and detection. j–m Western blots of membrane GLUT1 expression assays in peritoneal macrophages treated with or without 500 ng/ml LPS in the presence or absence of ML133 (25 μM), elevated [K+]e (50 mM) or gramicidin (1.25 μM) for 2 h (left panel, representative result; right panel, statistics of bands intensity). Two-tailed unpaired Student’s t-test. The FACS data are representative of three independent experiments. Gating strategies for FACS were shown in Supplementary Fig. 10. Source data are provided as a Source Data file.