Fig. 6.

CBD regulates progressive touch neuron defects through sir-2.1. A Lifespan analysis of WT worm strains at 20 ℃ fed with empty vector RNAi bacteria (EV) and sir-2.1 RNAi, treated with CBD or untreated. p values were determined using a log-rank test. B Lifespan analysis of WT strains and aak-2(ok524) mutant strains at 20 ℃ treated with CBD or untreated. p values by log-rank test. C Fluorescence images of autophagic flux in adult day 1 expressing rgef-1p::mCherry::GFP::lgg-1 in nerve-ring neurons. Worms fed with EV and sir-2.1 RNAi are treated with or without CBD. Scale bar = 25 μm. D, E Quantification of autophagosome (AP) punctae with mCherry/GFP signals and autolysosome (AL) punctae with only mCherry signals. Values are mean ± SEM of ≥ 15 animals combined from three independent experiments. *p ≤ 0.05 compared with control group by one-way ANOVA. F Fluorescence images of age-dependent defects of the ALM soma and PLM processes in worms fed with control EV or sir-2.1 RNAi and treated with CBD or untreated. Scale bar = 25 µm. D Quantification of ALM neurons with defective soma in worms fed with EV and sir-2.1 RNAi, treated with CBD or untreated, in day 1 and 8 adults. G Quantification of the PLM process defective in worms fed with EV and sir-2.1 RNAi and treated with or without CBD in day 1 and 8 adults. Error bars are SEs of proportions. At least 30 neurons were scored per time point. ns, not significant. *p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001