Table 1.
Genes differentially expressed by age in visceral fat-resident γδ T cells
| Gene symbol | Gene name | Function | Log 2 fold change | Linear fold change | p value |
|---|---|---|---|---|---|
| Upregulated in aged γδ T cells | |||||
| C6 | Complement component 6 | Complement system, phagocyte stimulation, bacterial pathogen clearance, NLRP3 inflammasome activation | 3.7 | 13.4 | 0.0031 |
| Cxcl13 |
Chemokine (CXC) ligand 13 |
T and B cell regulation and chemoattractant, inflammatory, infectious, and lymphoproliferative diseases | 3.3 | 9.6 | 0.0008 |
| Prg2 | Proteoglycan 2 | Immune hypersensitivity reactions, neutrophil and macrophage activation | 3.2 | 9.3 | 0.0067 |
| IL5ra | Interleukin 5 receptor, alpha | Eosinophil accumulation, biomarker and pharmacogenetic factor in asthma, Treg proliferation | 3.2 | 9.1 | 0.0044 |
| Ctla4 | Cytotoxic T-lymphocyte-associated protein 4 | Naïve T-cell activation, T cell motility and homeostasis | 2.5 | 5.7 | 0.0081 |
| Marco |
Macrophage receptor with collagenous structure |
Pattern recognition receptor, phagocytosis, pathogen clearance, facilitates ATM lipid uptake | 2.3 | 4.8 | 0.0098 |
| Ccl8 | Chemokine (C–C) ligand 8 (MCP-2) | Chemoattractant for multiple immune cells, promotes inflammation | 2.1 | 4.2 | 0.0123 |
| Il10 | Interleukin 10 | Anti-inflammatory cytokine, M2 polarization in adipose tissue, promotes insulin resistance, thermogenesis | 1.8 | 3.5 | 0.0138 |
| Downregulated in aged γδ T cells | |||||
| Col4a1 | Collagen alpha-1(IV) chain | Angiogenesis, regulates HIF-1α and VEGF, expression in AT positively correlates with insulin resistance | − 3.9 | − 14.9 | 0.0029 |
| Xcl1 | Chemokine (C) ligand (lymphotactin) | Chemotaxis and activation of lymphocytes, adipose stem cell homing | − 3.2 | − 9.4 | 0.0138 |
| Col1a1 | Collagen alpha-1 (I) chain | Component of type I collagen, make up most connective tissues | − 3.1 | − 8.7 | 0.0095 |
| Cfd | Complement factor D | Stimulates glucose transport, inhibits lipolysis, promotes adipocyte differentiation | − 3.1 | − 8.5 | 0.0021 |
| Col3a1 | Collagen alpha-1 (III) chain | Component of type III collagen, platelet aggregation, blood clotting | − 2.9 | − 7.4 | 0.0139 |
| Lbp | Lipopolysaccharide binding protein | Acute phase immune reactions, presents LPS to immune cell surface | − 2.3 | − 4.8 | 0.0039 |
| Thbs1 | Thrombospondin 1 | Involved in endothelial cell adhesion and angiogenesis, enhances preadipocyte proliferation | − 2.0 | − 4.0 | 0.0052 |
| Klrd1 | Killer cell lectin-like receptor subfamily D, member 1 (CD94) | Binding with HLA-E on target cells facilitates activation and expansion of NK and T cell subsets, overexpressed in γδT-cell lymphoma | − 1.9 | − 3.8 | 0.0150 |
| Il6st | Interleukin 6 signal transducer (glycoprotein 130) | Cytokine signaling, modulates pro- and anti-inflammatory pathways | − 1.8 | − 3.6 | 0.0026 |
| Ccl17 | Chemokine (C–C) ligand 17 | Induces T cell chemotaxis and activation, drives inflammation and pain through GM-CSF signaling | − 1.8 | − 3.5 | 0.0069 |