Fig. 5.

Long-term exercise training preserves mitochondrial dynamics and mitochondrial morphology in TgF344-AD rats. A Representative confocal microscopy images of Tom20 staining captured from the cortex and hippocampus. Images of Tom20 fluorescent staining were further processed by ImageJ software into images with total particles, small particles (size < 1.5 μm), and continuous structure (size > 2 μm). n = 8. Scale bar = 10 μm. B Representative confocal microscopy images and Western blot results of MFN1 in the cortex and hippocampus. n = 3. C Western blot analysis of Fis1 (n = 3–4). D Representative transmission electron micrograph of mitochondria. Scale bar = 50 nm. E Representative confocal microscopy images and Western blot results of PGC-1α in the cortex and hippocampus (n = 3). Scale bar = 10 μm. Data are presented as mean ± SEM. *P < 0.05 versus WT group, ^#P < 0.05 versus AD group