Fig. 1.

Effect of diabetes on blood glucose, body weight, HbA1c levels, and pancreatic insulin content in CB₂^−/− mice and JWH-133 treated WT diabetic mice. A Time scheme of diabetes induction. B Treatment scheme with the selective CB2 receptor agonist JWH133. C and D Blood glucose was monitored during the course of the study as described in the methods section. E and F Body weights were measured initially and after induction of diabetes periodically, and the data shown indicates the baseline and the final body weights in the respective groups. At the end of the study, EDTA whole blood was collected, and the glycosylated hemoglobin (HbA_1C) levels were determined as described in the Methods section. After the completion of the study, the pancreas was excised snap frozen in liquid nitrogen, and later, the insulin levels were determined using ELISA as described in the Methods section. #P < 0.05 vs. vehicle/JWH-133 (initial vs. end); *P < 0.05 vs. diabetes/diab + JWH-133 (initial vs. end), n = 10–12/group. #P < 0.05 vs. WT-vehicle/CB2^−/− vehicle (initial vs. end); *P < 0.05 vs. WT diabetes/CB2^−/− diabetes (initial vs. end), n = 10–12/group