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. 2022 Jun 8;13:903333. doi: 10.3389/fpls.2022.903333

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Copyright © 2022 Xue, Huang, Zhang, Lin, Zhong, Zhao, Gao and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of the MrGST1 by MrMYB1.1. (A) Yeast one-hybrid interaction between MrMYB1.1 and MrGST1 promoter. (B) Effects of MrMYB1.1 (+/− MrbHLH1) on the promoter activity of MrGST1 measured by dual-luciferase assays. (C) Schematic diagrams of the MrGST1 promoter with eight MYB binding site (MBS) motif mutations. (D) Effects of MrMYB1.1 (+/− MrbHLH1) on the activity of original and mutated promoters of MrGST1 measured by dual-luciferase assays. (E) Yeast one-hybrid interaction between MrMYB1.1 and three tandem repeats of MBS4 motif of MrGST1 promoter. Data were means (±SE) from three independent biological replicates. Asterisks (^**) indicate significant differences at p < 0.05 level.