Figure 2.

ATR localization to the midbody is dependent on ATRIP and ETAA1 but independent of DNA, RPA, and TOPBP1

(A) ATR-T1989p localizes to midbodies in all cytokinetic cells irrespective of the presence or absence of DAPI bridges.

(B) ATR-T1989p localizes to midbodies in all cytokinetic cells irrespective of the presence or absence of LAP2β bridges.

(C) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of ATRIP or RPA2.

(D) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of ATRIP or RPA2 (∗∗∗p = 0.001, ns; Student’s t-test).

(E) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of RPA1.

(F) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of RPA1 (∗p = 0.0382; Student’s t-test).

(G) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of TOPBP1.

(H) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of TOPBP1 (ns; Student’s t-test).

(I) Localization of ATR-T1989p to late cytokinetic midbodies upon depletion of ETAA1.

(J) Quantification of ATR-T1989p intensity at late cytokinetic midbodies upon depletion of ETAA1 (∗∗∗p = 0.0004; Student’s t-test). All dot plots represent data from at least three independent biological replicates, each replicate is represented by a different symbol. All data have been normalized to the control sample. Data are represented as mean ± SD of the individual replicates. HeLa cells were used in A–J. All representative images shown were selected from one of at least three independent experiments and stained as indicated. All scale bars are 10 μm, all enlarged image scale bars are 2 μm. See also Figure S2.