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. 2022 Apr 27;63(7):100219. doi: 10.1016/j.jlr.2022.100219

Table 1.

A list of MS/MS approaches for characterization of complex lipids at the levels of C=C location and sn-position

Structure Information MS Approach Lipids Samples Reference
C=C locations Shotgun PB-MS/MS FA and GPL Lipid extracts (58)
LC-PB-MS/MS FA and GPL Lipid extracts, plasma samples (59, 60, 61, 65)
MALDI-PB-MS/MS GPL and glycosphingolipid, Mouse brain tissue (70, 114)
UVPD-MS PC, cardiolipin, FA, sphingolipid, SM, TG E. coli and human papillary thyroid carcinoma extracts (35, 36, 38, 115)
LC-UVPD-MS FA and GPL Bovine liver and porcine brain extracts (49, 63)
DESI-UVPD-MS PC Brain tissue, lymph node tissue (40)
OzID-MS GPL, SM, TG Lipid extracts (27, 31)
LC-OzID-MS PC, PE, SM Human plasma (32)
epoxidation-MS/MS FA and GPL Bovine liver extract, human plasma, yeast extract, serum samples (63, 72, 73, 76)
epoxidation-LC-MS/MS FA and GPL Human serum (76)
epoxidation-DESI-MS/MS FA and GPL Human serum, mouse adipocytes, and cancer tissue sections (76)
epoxidation-MALDI-MS/MS FA Cancer tissue samples, human cell lines (63)
HAD/OAD- MS/MS PC and LPC Standards (56)
sn-positions (+Na+) PB-MS3 GPL Human breast cancer cells (66)
(+Na+) OzID-MS3 GPL Cow brain and kidney extracts (96, 98)
(+Na+) UVPD-MS3 GP Bovine liver and porcine brain extracts (34)
(+HCO3-) PB-MS3 PC Tissue samples of human breast cancer (100)
(+Fe2+) UVPD-MS3 PC Lipid extracts of mouse pancreas tissue (102)
EIEIO PC Egg yolk extracts (42)

FA, fatty acid; GPL, glycerophospholipid; TG, triglyceride; EIEIO, electron impact excitation of ions from organics; UVPD, ultraviolet photodissociation; OzID, ozone-induced dissociation; OAD, oxygen attachment dissociation; PB, Paternò-Büchi.