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. 2022 May 11;63(6):100225. doi: 10.1016/j.jlr.2022.100225

Fig. 6.

Fig. 6

SPHK1 localizes to the early endosomes, SPHK2 localizes to the cytosol and nucleus, and SGPL1 localizes to the ER. A: eGFP-SPHK1, (F) eGFP-SPHK2, and (K) SGPL1-eGFP were transfected into HeLa cells along with RFP-tagged organelle markers (supplemental Table S1) for (B) early endosomes (EE) or (L) ER, or with mCherry2-C1 plasmid for (G) cytosol. C, H, and M: Nuclei (blue) were stained by NucBlue Live Cell Stain ReadyProbeReagent (Hoechst 33342). Cells were examined by confocal fluorescence microscopy. D, I, and N: Merged images. E, J, and O: Colocalization of fluorescent markers was determined by Manders overlap coefficient using ZEN Blue software (ZEN 2012 SP5). n=20–30 cells. RFP, red fluorescent protein.