Skip to main content
. 2022 Jun 3;25(7):104520. doi: 10.1016/j.isci.2022.104520
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-mouse CD11b-FITC (M1/70) In-house
Anti-mouse CD11b-AF700 (M1/70) BD Biosciences Cat# 557960, RRID: AB_396960
Anti-mouse CD11b-APC (M1/70) BioLegend Cat# 10128
Anti-mouse CD11b-PE-e610 eBioscience Cat# 61-0112-82, RRID: AB_2574528
Anti-mouse CD11c-PE-Cy7 (HL3) BD Biosciences Cat# 558079, RRID: AB_647251
Anti-mouse CD16/32 eBioscience Cat# 14-0161-86, RRID: AB_467135
Anti-mouse CD19-FITC (1D3) BD Biosciences Cat# 561740, RRID: AB_10893811
Anti-mouse CD45-APC-e780 (30-F11) eBioscience Cat# 47-0454-82, RRID: AB_1272175
Anti-mouse CD45-e506 eBioscience Cat# 69-0454-82, RRID: AB_2637105
Anti-mouse CD64-PerCP-e710 eBioscience Cat# 46-0641-82, RRID: AB_2735016
Anti-mouse F4/80-PE (CI:A3-1) Cedarlane labs Cat# CL8940PE, RRID: AB_10060422
Anti-mouse F4/80-A647 (T45-2342) BD Biosciences Cat# 565853, RRID: AB_2744474
Anti-mouse Gr1-PE (RB6-8C5) BD Biosciences Cat# 553128, RRID: AB_394644
Anti-mouse Ly6C-Biotin (AL-21) BD Biosciences Cat# 557359, RRID: AB_396663
Anti-mouse Ly6C-BV605 (AL-21) BD Biosciences Cat# 563011, RRID: AB_2737949
Anti-mouse Ly6G-AF700 (1A8) BD Biosciences Cat# 561236, RRID: AB_10611860
Anti-mouse Ly6G-PE (1A8) BD Biosciences Cat# 551461, RRID: AB_394208
Anti-mouse Ly6G-APC (1A8) BD Biosciences Cat# 560599, RRID: AB_172756
Anti-mouse MHCII-BV786 (M5/114.15) BD Biosciences Cat# 742894, RRID: AB_2734759
Anti-mouse TCRβ-e450 (H57-597) eBioscience Cat# 48-5961-82, RRID: AB_11039532
Anti-mouse Iba-1 Wako, Japan N/A
Goat anti-rabbit IgG H&L-AF568 Life Technologies N/A
Anti-rabbit IgG-biotin Dako Cytomation N/A

Chemicals, peptides, and recombinant proteins

Sodium pentobarbitone Virbac, Peakhurst, Australia N/A
Liquid DAB+ Substrate Chromagen System Dako K3468
Collagenase from clostridium histolyticum Sigma Aldrich C5138
Deoxyribonuclease I Sigma Aldrich DN25
LIVE/DEAD Fixable Aqua Invitrogen L34957
Fixable Viability eFluor 780 eBioscience 65-0865-14
Sodium thioglycollate Sigma Aldrich T0632
FITC-isolectin B4 (from Bandeiraea simplicifolia (Griffonia simplicifolia)) Sigma Aldrich L2895
Hoescht33342 ThermoFisher H1399
Alexa 488-Phalloidin Invitrogen A12379
DePeX mounting medium VWR International Ltd., Poole, England SERA18243.01
Mouse recombinant KC (CXCL1) Peprotech 250-11
Mouse recombinant CSF-1 Peprotech 315-02
Mouse recombinant IL-4 eBiosciences 14-8041-80
Mouse recombinant IL-13 eBiosciences 14-8131-80
Mouse recombinant IFN-γ eBiosciences 14-8311-63
LPS-EB Ultrapure Invivogen Tlrl-3pelps

Critical commercial assays

Vectastain ABC kit, standard Vector Laboratories PK-4000
Mouse albumin ELISA kit Bethyl Laboratories, Mongomery, TX, USA E99-134

Experimental models: Organisms/strains

WT C57Bl/6 In-house breeding, Animal Research Laboratories, Clayton, Australia N/A
Cd82−/− C57Bl/6 In-house breeding, Animal Research Laboratories, Clayton, Australia N/A
L. mexicana In-house propagation N/A
L. mexicanaTurboRFP In-house propagation N/A

Oligonucleotides

Arginase 1 (Arg1)-forward primer: CCACAGTCTGGCAGTTGGAA Bioneer Pacific N/A
Arginase 1 (Arg1)-reverse primer: GCATCCACCCAAATGACACA Bioneer Pacific N/A
Arginase 1 (Arg1)-probe:
FAM-TGGCCACGCCAGGGTCCAC-TAMRA
Bioneer Pacific N/A
Mannose receptor (Mrc1)-forward primer: AATACCTTGAACCCATTTATCATTCC Bioneer Pacific N/A
Mannose receptor (Mrc1)-reverse primer: GCATAGGGCCACCACTGATT Bioneer Pacific N/A
Mannose receptor (Mrc1)-probe:
FAM-CGATGTGCCTACCGGCTGCCC-TAMRA
Bioneer Pacific N/A
Nitric oxide synthase (Nos2)-forward primer: GGGCAGCCTGTGAGACCTT Bioneer Pacific N/A
Nitric oxide synthase (Nos2)-reverse primer: TGCATTGGAAGTGAAGCGTTT Bioneer Pacific N/A
Nitric oxide synthase (Nos2)-probe:
FAM- TCCGAAGCAAACATCACATTCAGATCCC-TAMRA
Bioneer Pacific N/A

Software and algorithms

FlowJo TreeStar Inc, OR, USA N/A
GraphPad Prism (version 6) San Diego, CA USA N/A
ImageJ NIH, MA, USA N/A

Other

PROOX 110 gas regulator Reming Bioinstruments Co., Redfield NY USA N/A
Nikon A1 laser scanning confocal microscope Nikon Instruments Inc, Melville, NY, USA N/A
Leica SP8 inverted confocal microscope Leica Microsystems Pty Ltd, NSW, Australia N/A
ZEISS LSM980 Axioplan 2 microscope ZEISS Australia, North Ryde, NSW N/A
LSR II Fortessa cell analyser BD Biosciences Australia N/A
Transwells 65 mm diameter 5.0 μm pore size DKSH 3421
Caliper Vernier, Digital LCD Westlab Pty Ltd 080322-0003