| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD11b-FITC (M1/70) | In-house | |
| Anti-mouse CD11b-AF700 (M1/70) | BD Biosciences | Cat# 557960, RRID: AB_396960 |
| Anti-mouse CD11b-APC (M1/70) | BioLegend | Cat# 10128 |
| Anti-mouse CD11b-PE-e610 | eBioscience | Cat# 61-0112-82, RRID: AB_2574528 |
| Anti-mouse CD11c-PE-Cy7 (HL3) | BD Biosciences | Cat# 558079, RRID: AB_647251 |
| Anti-mouse CD16/32 | eBioscience | Cat# 14-0161-86, RRID: AB_467135 |
| Anti-mouse CD19-FITC (1D3) | BD Biosciences | Cat# 561740, RRID: AB_10893811 |
| Anti-mouse CD45-APC-e780 (30-F11) | eBioscience | Cat# 47-0454-82, RRID: AB_1272175 |
| Anti-mouse CD45-e506 | eBioscience | Cat# 69-0454-82, RRID: AB_2637105 |
| Anti-mouse CD64-PerCP-e710 | eBioscience | Cat# 46-0641-82, RRID: AB_2735016 |
| Anti-mouse F4/80-PE (CI:A3-1) | Cedarlane labs | Cat# CL8940PE, RRID: AB_10060422 |
| Anti-mouse F4/80-A647 (T45-2342) | BD Biosciences | Cat# 565853, RRID: AB_2744474 |
| Anti-mouse Gr1-PE (RB6-8C5) | BD Biosciences | Cat# 553128, RRID: AB_394644 |
| Anti-mouse Ly6C-Biotin (AL-21) | BD Biosciences | Cat# 557359, RRID: AB_396663 |
| Anti-mouse Ly6C-BV605 (AL-21) | BD Biosciences | Cat# 563011, RRID: AB_2737949 |
| Anti-mouse Ly6G-AF700 (1A8) | BD Biosciences | Cat# 561236, RRID: AB_10611860 |
| Anti-mouse Ly6G-PE (1A8) | BD Biosciences | Cat# 551461, RRID: AB_394208 |
| Anti-mouse Ly6G-APC (1A8) | BD Biosciences | Cat# 560599, RRID: AB_172756 |
| Anti-mouse MHCII-BV786 (M5/114.15) | BD Biosciences | Cat# 742894, RRID: AB_2734759 |
| Anti-mouse TCRβ-e450 (H57-597) | eBioscience | Cat# 48-5961-82, RRID: AB_11039532 |
| Anti-mouse Iba-1 | Wako, Japan | N/A |
| Goat anti-rabbit IgG H&L-AF568 | Life Technologies | N/A |
| Anti-rabbit IgG-biotin | Dako Cytomation | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Sodium pentobarbitone | Virbac, Peakhurst, Australia | N/A |
| Liquid DAB+ Substrate Chromagen System | Dako | K3468 |
| Collagenase from clostridium histolyticum | Sigma Aldrich | C5138 |
| Deoxyribonuclease I | Sigma Aldrich | DN25 |
| LIVE/DEAD Fixable Aqua | Invitrogen | L34957 |
| Fixable Viability eFluor 780 | eBioscience | 65-0865-14 |
| Sodium thioglycollate | Sigma Aldrich | T0632 |
| FITC-isolectin B4 (from Bandeiraea simplicifolia (Griffonia simplicifolia)) | Sigma Aldrich | L2895 |
| Hoescht33342 | ThermoFisher | H1399 |
| Alexa 488-Phalloidin | Invitrogen | A12379 |
| DePeX mounting medium | VWR International Ltd., Poole, England | SERA18243.01 |
| Mouse recombinant KC (CXCL1) | Peprotech | 250-11 |
| Mouse recombinant CSF-1 | Peprotech | 315-02 |
| Mouse recombinant IL-4 | eBiosciences | 14-8041-80 |
| Mouse recombinant IL-13 | eBiosciences | 14-8131-80 |
| Mouse recombinant IFN-γ | eBiosciences | 14-8311-63 |
| LPS-EB Ultrapure | Invivogen | Tlrl-3pelps |
| Critical commercial assays | ||
| Vectastain ABC kit, standard | Vector Laboratories | PK-4000 |
| Mouse albumin ELISA kit | Bethyl Laboratories, Mongomery, TX, USA | E99-134 |
| Experimental models: Organisms/strains | ||
| WT C57Bl/6 | In-house breeding, Animal Research Laboratories, Clayton, Australia | N/A |
| Cd82−/− C57Bl/6 | In-house breeding, Animal Research Laboratories, Clayton, Australia | N/A |
| L. mexicana | In-house propagation | N/A |
| L. mexicanaTurboRFP | In-house propagation | N/A |
| Oligonucleotides | ||
| Arginase 1 (Arg1)-forward primer: CCACAGTCTGGCAGTTGGAA | Bioneer Pacific | N/A |
| Arginase 1 (Arg1)-reverse primer: GCATCCACCCAAATGACACA | Bioneer Pacific | N/A |
| Arginase 1 (Arg1)-probe: FAM-TGGCCACGCCAGGGTCCAC-TAMRA |
Bioneer Pacific | N/A |
| Mannose receptor (Mrc1)-forward primer: AATACCTTGAACCCATTTATCATTCC | Bioneer Pacific | N/A |
| Mannose receptor (Mrc1)-reverse primer: GCATAGGGCCACCACTGATT | Bioneer Pacific | N/A |
| Mannose receptor (Mrc1)-probe: FAM-CGATGTGCCTACCGGCTGCCC-TAMRA |
Bioneer Pacific | N/A |
| Nitric oxide synthase (Nos2)-forward primer: GGGCAGCCTGTGAGACCTT | Bioneer Pacific | N/A |
| Nitric oxide synthase (Nos2)-reverse primer: TGCATTGGAAGTGAAGCGTTT | Bioneer Pacific | N/A |
| Nitric oxide synthase (Nos2)-probe: FAM- TCCGAAGCAAACATCACATTCAGATCCC-TAMRA |
Bioneer Pacific | N/A |
| Software and algorithms | ||
| FlowJo | TreeStar Inc, OR, USA | N/A |
| GraphPad Prism (version 6) | San Diego, CA USA | N/A |
| ImageJ | NIH, MA, USA | N/A |
| Other | ||
| PROOX 110 gas regulator | Reming Bioinstruments Co., Redfield NY USA | N/A |
| Nikon A1 laser scanning confocal microscope | Nikon Instruments Inc, Melville, NY, USA | N/A |
| Leica SP8 inverted confocal microscope | Leica Microsystems Pty Ltd, NSW, Australia | N/A |
| ZEISS LSM980 Axioplan 2 microscope | ZEISS Australia, North Ryde, NSW | N/A |
| LSR II Fortessa cell analyser | BD Biosciences Australia | N/A |
| Transwells 65 mm diameter 5.0 μm pore size | DKSH | 3421 |
| Caliper Vernier, Digital LCD | Westlab Pty Ltd | 080322-0003 |