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. 2022 Jun 8;10:854918. doi: 10.3389/fchem.2022.854918

TABLE 2.

Nucleophilic rate constant values for the reaction between FDNB with biothiol series in aqueous media at 25°C.

Biothiol pKa FDNB kN (sM)−1
L-Cysteine ethyl ester 6.50 5.45 ± 0.17
Cysteine 8.10 21.32 ± 0.66
Glutathione 8.75 66.90 ± 1.62
N-Acetylcysteine 9.50 95.63 ± 3.28