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. 2022 Jun 8;13:910217. doi: 10.3389/fphar.2022.910217

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Copyright © 2022 Ji, Shi, Li, Hou, Yang, Liu, Duan, Liu, Chen and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of THSWD treatment on Mitochondrial structure and autophagy proteins. (A) Ultrastructural changes in mitochondria. Scale bar = 500 nm. The red arrows marked the mitochondria, and the green arrows marked the many autophagic vesicles that wrapped around the mitochondria and fused with the lysosomes in the MCAO/R + THSWD group. (B,C) Immunofluorescence staining of LC3B and Beclin1 (×400). Scale bar = 50 μm. The mean fluorescence intensities (MFI) of LC3B and Beclin1 are quantified in the right-hand histogram. n = 4 per group. ^* p < 0.05, ^** p < 0.01 versus sham group, ^# p < 0.05, ^### p < 0.001 versus MCAO/R group, ^&&& p < 0.001 versus MCAO/R + THWSD group.