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Representative graph showing the plasma membrane stability of EGFR after 20 min of EGF stimulation (50 ng/mL, 37°C) in ESCRT-depleted HeLa cells (shESCRT) compared to control cells (HeLa NT)
EGFR levels after stimulation (t=20 min) are normalized to that in the unstimulated plate (t=0 h) and plotted as the percentage remaining at the cell surface. Values represent the average mean +-SEM.
