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. 2022 Jun 6;15:100309. doi: 10.1016/j.mtbio.2022.100309

Fig. 10.

Fig. 10

Cell and particle interaction on the GEL/dbPTs composite scaffolds at 14 days of incubation period. (A) SEM images of the MC3T3-E1 cells (black arrows) that interacted with particles (yellow arrow) on GEL/dbPTs scaffolds. Scale bar: 20 ​μm (left), 5 μm (right). (B–E) Multiphoton microscopy images, cyan ​= ​DAPI, red ​= ​F-Actin, blue ​= ​SHG. (B) 3D view of cells growing on GEL/dbPTs-composite scaffolds (yellow arrows indicate particles in blue). Scale bar: 100 ​μm (scale bar indicated by red cage) (C) Top of scaffolds covered with cell layer. Maximum intensity projection. Scale bar: 100 ​μm. (D) 3D view of cells growing on porous scaffolds and around dbPTs in blue (yellow stars and arrows indicate pore and particle). Scale bar: 50 ​μm ​(E) Cells interacting with decellularized bone particles (yellow arrows). Maximum intensity projection. Scale bar: 50 ​μm.