Fig. 2.

Results of the decellularization process to obtain dbPTs. (A) Histological hematoxylin & eosin staining showing cell nuclei in native bone tissue before decellularization and empty lacunae after decellularization. Scale bar: 100 ​μm. (B) SEM images of the bone tissue showing also magnified images of collagen structures. Scale bar: 100 ​μm, 5 ​μm (insets). (C) Total DNA and (D) collagen content of the bone tissues before and after decellularization. (DE: decellularization). Data are shown as mean ​± ​SD. ∗∗∗p ​< ​0.001 indicates statistically significant difference of means by one way ANOVA test.