Fig. 8.

In vitro cytocompatibility assessment of the GEL/dbPTs composite scaffolds. (A) Schematic illustration of the cultured GEL/dbPTs scaffolds with MC3T3-E1 cells. (B) Quantification of the extracellular LDH during 21 days of incubation with no significant differences (ns) between groups on each time point (n ​= ​6). (C) Cell viability assay of MC3T3-E1 cells on the GEL/dbPTs scaffolds (n ​= ​6), normalized to TCP control on first day of incubation. Statistically significant differences were determined between groups depending on each time point. All data are represented as mean ​± ​SD. ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001 indicate statistically significant difference among means. (D) Fluorescence microscopy images of MC3T3 cells on the scaffolds cultured for 14 days. Calcein AM (green), PI (red), DAPI (blue) staining represent live, dead cells and cell nuclei respectively. (Scale bars: 100 ​μm).