Table 2.
INSRA/IGF1R ratio increases in GBM stem cells
| Gene | hNSC1 | hNSC2 | GSC1 | GSC2 | GSC3 | GSC4 | GSC5 | GSC6 |
|---|---|---|---|---|---|---|---|---|
| INSR | 11.4395 | 2.97384 | 6.75019 | 3.62512 | 7.17077 | 3.15267 | 3.06491 | 4.17927 |
| IGF1R | 57.0076 | 24.9964 | 6.06387 | 10.2188 | 12.2469 | 5.49724 | 3.69863 | 21.8827 |
| INSR/IGF1R | 0.20 | 0.12 | 1.11 | 0.35 | 0.59 | 0.57 | 0.83 | 0.19 |
| Average (Avg.) of INSR/IGF-1R ratios | 0.16 | 0.61 | ||||||
| Fold change | 1 | 3.8a | ||||||
INSR and IGF1R mRNA levels were quantified from GBM neurospheres from deep sequencing using Roche 454/GS FLX Sequencing Technology. Exon 11 (present only in INSRB) was undetectable in the human NSC (hNSC) populations glioma stem cells (GSC) and hNSC. Thus, the INSR in NSCs corresponds to INSRA (glioma stem cells: n = 6, normal human neural stem cells: n = 2). Data represent mean values of RNA-seq reads.
a
Increased expression.