Figure 2.
Cortical brain organoids generate cortical plate neurons and glial cells
(A–C) Images of week 5 cortical organoids showing the presence of TBR1 and CTIP2 cortical plate neurons.
(D) Cortical organoid diameter and significant size increase with days in culture (n = 30 organoids, N = 3 differentiation batches, mean ± SD, unpaired two-tailed t test).
(E–H) Organoids showing CTIP2- and SATB2-positive cells (E and F; high-magnification image of inset in E shown in F) and S100B and GFAP glial cells (G and H).
(I) Percentage of cells expressing TUJ1 and GFAP per area (mm2) in 11-week-old cells (n = 10 images from 2 or 3 organoids, N = 3 differentiation batches, mean ± SD, unpaired two-tailed t test).
(J–L). Week 15 cortical organoid showing GABA- and CALRETININ-positive inhibitory neurons.
(K) shows high-magnification image of inset in (J).
Scale bars, 15 μm (K), 50 μm (F), 75 μm (A–C), and 100 μm (E, G, H, J, and L).