Figure 1.

Amino acid mutations in the RBD of the SARS-CoV-2 spike protein detected via NGS of respiratory samples from patient A (A), patient B (B), and patient C (C) over time since the first positive sample. Each column represents 1 sample, sample ID corresponds to sample ID in Supplementary Table 2. Light-gray boxes denote positions where >75% of reads are the wild-type allele, dark-gray boxes denote positions where >75% of reads are the mutant allele, and striped boxes denote mixed positions, with the number representing the proportion of reads with the mutant allele detected. White boxes are positions where there is no sequencing coverage. More details on genome coverage, read depth, and accession numbers are provided in Supplementary Table 2. Abbreviations: NGS, next-generation sequencing; RBD, receptor binding domain; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2.