TABLE 1.

Primer sets and probes used for human enteric virus detection

Virus	Primer	Probe	Sequence	Locationa	Fragment length (bp)	Reference
AV	Mon2		GCTTCTGATTAAATCAATTTT	6776–6797		40
	Mon1		CGAGTAGGATCGAGGGTA	6709–6727	89	40
		Ap	ACCATTTAAAATTGATTTAATC	6767–6786		36
EV	E1		TCCGGCCCCTGAATGCGG	446–463		7
	E2		CACCGGATGGCCAATCCAAT	623–642	196	7
		Ep	ACACGGACACCCAAAGTAGTCGGTTGG	533–559		7
	Primer 2		CAAGCACTTCTGTTTCCCCGG	162–182		26
	Primer 3		ATTGTCACCATAAGCAGCCA	577–596	434	26
HAV	H1		GGAAATGTCTCAGGTACTTTCTTTG	2389–2413		43
	H2		GTTTTGCTCCTCTTTATCATGCTATG	2167–2192	247	43
		Hp	TCAACAACAGTTTCTACAGA	2233–2252		43
NLV	P110		GTAAAACGACGGCCAGACDATYC	4865–4884		28
	NVP36		CAGGAAACAGCTATGACATAAAAG	4487–4501	397	25
	P69		GGCCTGCCATCTGGATTGCC	4733–4752	150	25
	NI		GAATTCCATCGCCCACTGGCT	4768–4788	116	21
	SR48		GTGAACAGCATAAATCACTGG	4766–4786	118	1
	SR50		GTGAACAGTATAAACCACTGG	4766–4786	118	1
	SR52		GTGAACAGTATAAACCATTGG	4766–4786	118	1
		NVP116	CTCTGTGCACTITCTGAAGT	4796–4815		28
		NVP117	ACCTTGTGTGCCATGTCTGA	4793–4812		28
		NVP118	CTATGTGCACTGTCAGGAGT	4796–4815		28
		SR47	ATGTCAGGGGACAGGTTTGT	4817–4836		1
		SR61	ATGTCGGGGCCTAGTCCTGT	4817–4836		1
RV	Beg9		GGCTTTAAAAGAGAGAATTTCCGTCTGG	1–28		19
	R4		GATCCTGTTGGCCATCC	376–392	392	17
		RFP5	GTATGGTATTGAATATACCAC	51–71		17
		G1	GTCACCATCATTGATTGAGTACTT	315–339		48
		G2	TTCATCATCTGAAATCTCATTTTTA	315–339		48
		G3	TGAATTATCATTTATTTCTGTTGCT	315–339		48
		G4	TTCAGTGTCACTAATTTGAGTTGGA	315–339		48

^aNucleotide positions are in reference to AV serotype 2 (L13745), poliovirus type 1 (Mahoney strain) (V011148), HAV strain HM175 (M16632), Norwalk virus genome (M87661), and RV serotype G1 strain Wa (M21843).