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. 2022 May 26;17(6):1366–1379. doi: 10.1016/j.stemcr.2022.05.001

Figure 2.

Figure 2

Ts21 iPSCs generate fewer CR+ interneurons and fewer COUP-TFII+ progenitors

(A) Interneuron progenitor differentiation protocol.

(B) Proportion of calretinin (CR) neurons/(NeuN+) differentiated from control and Ts21 progenitors at days 74 and 91. ∗p = 0.023 using unpaired t test with two-stage step up (Benjamini, Krieger, and Yekutieli).Control is black and Ts21 is red.

(C) Immunofluorescence images of NKX2.1+ and COUP-TFII+ nuclei in control and Ts21 neural progenitor cells (NPCs).

(D) Proportion of NKX2.1+ COUP-TFII- cells (MGE), NKX2.1+ COUP-TFII+ cells (caudal MGE), and NKX2.1- COUP-TFII+ cells (CGE) in control and Ts21 NPCs. ∗∗p < 0.001 using unpaired t test, N = 4 cell lines.

(E) Expression of NKX2.1 and COUP-TFII in human fetal control and DS brain (14–17 weeks gestation, dorsolateral forebrain) (Olmos-Serrano et al., 2016). Control is black and Ts21 is red.

(F) NKX2.1+/EdU+ and COUP-TFII+/EdU+ proliferating cells in the Ts21 cells compared with controls. ∗p < 0.05 using unpaired t test, N = 4.