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. 2022 May 26;17(6):1366–1379. doi: 10.1016/j.stemcr.2022.05.001

Figure 5.

Figure 5

Ts21 ventral progenitors have decreased WNT and increased GLI3 expression

(A and B) Quantitative PCR of SHH pathway genes (A) WNT signaling genes (B) in two iPSC lines of Ts21 and isogenic control lines.

(C) Expression of SHH and WNT pathway genes in human fetal control and DS brain (14–17 weeks gestation, dorsolateral forebrain, Olmos-Serrano et al., 2016).

(D) Quantitative PCR of GLI genes in progenitor cells and in human fetal control and DS brain (Olmos-Serrano et al., 2016). Statistical significance was determined by one-sample t test on ddCt values. ∗p < 0.05, N = 3 for each gene/line.

(E) The effects of activation of WNT via addition of a WNT agonist (CHIR, 0.4 and 1.2 μm) with SHH on COUP-TFII/NR2F2 gene expression, proportion of COUP-TFII+ cells, and GLI3 expression. Statistical significance was determined by one-sample t test on ddCt values. ∗p < 0.05, N = 3 replicates in one pair of isogenic control and Ts21 cells.