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. 2022 Jan 6;12(6):2887–2904. doi: 10.1016/j.apsb.2021.12.023

Figure 7.

Figure 7

GPS promoted the interaction between DDB2 and PAQR3 to increase PAQR3 ubiquitin-mediated degradation in vivo. (A) and (B) Paqr3 and Ddb2 mRNA level in liver tissue (n = 3). (C) Representative Western blots and densitometric analysis of DDB2 protein level in liver tissue (n = 3). (D) Immunofluorescence staining presents the distribution and co-localization of PAQR3 and DDB2 in liver tissue. Scale bar = 100 μm. (E) A co-immunoprecipitation assay was performed to detect the interaction between DDB2 and PAQR3 in liver tissue. (F) GPS promoted the polyubiquitination of PAQR3 in liver tissue. Data are presented as mean ± SD, all experiments were performed at least three times with similar results. ∗∗∗P < 0.001, ∗∗P < 0.01 vs. control group; ###P < 0.001, ##P < 0.01 vs. diabetes group; ns: no significance.