Figure 2.

FGFR3 protein is expressed by PGCs in the prenatal human ovary

(A) Schematic representation of germ cell differentiation in prenatal ovaries. TFAP2C+/VASA^low PGCs mostly reside at the cortex of the ovary. With time, PGCs differentiate and advance toward meiosis by upregulating expression of VASA and silencing TFAP2C. Right-hand side: immunostaining of ovarian tissues at weeks 7, 10, and 14 with TFAP2C (magenta), FGFR3 (yellow), VASA (cyan), and DAPI (blue).

(B) Quantification of the percentage of FGFR3+ and FGFR- cells that express TFAP2C and low levels of VASA (50 cells were counted for each sample).

(C) Quantification of FGFR3+ and FGFR3- cells that are negative for TFAP2C and positive for VASA (n = 3 samples for each category; 50 cells were counted from each sample).

(D) Immunostaining of a 14 week ovary with TFAP2C (magenta), FGFR3 (yellow), VASA (cyan), and DAPI (blue).

(E) Nuclei size of VASA+/FGFR3+ and VASA+/FGFR3- ovarian germ cells (25 cells were measured for each category from a 14 week ovary). ^∗∗∗p < 0.001. Statistical significance was assessed by Wilcoxon test.