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. 2022 May 19;17(6):1268–1278. doi: 10.1016/j.stemcr.2022.04.015

Figure 4.

Figure 4

FGFR3 mRNA and protein are expressed by PGCs before gonadal colonization

(A) Immunostaining of a week 5 embryo showing the genital ridge. OCT4 (magenta), DAZL (cyan), FGFR3 (yellow) and DAPI (blue). n = 1 sample.

(B) Expression of FGFR3 in female and male PGCs at weeks 4 and 5 from Li et al. (2017) dataset.

(C) Week 4 and 5 germ cells and somatic cells depicted on a UMAP displaying expression of POU5F1 (OCT4), NANOG, and FGFR3.

(D) Day 4 PGCLCs in three replicates (Chen et al., 2019) differentiated from UCLA1 hESCs and expression of diagnostic markers: NANOS3 for PGCLCs, SOX17 for endoderm cells, HAND1 for mesoderm cells and SOX17/NANOS3 double positive cells for PGCLCs.

(E) Annotation of D4 aggregate cells based on marker expression from (D).

(F) Expression of FGFR3 depicted at t-SNE maps and violin plots (G).

(H) UCLA1 female hESCs differentiated for 4 days to create aggregates and immunostained for OCT4 (cyan), FGFR3 (yellow), TFAP2C (magenta), and DAPI (blue) (n = 2 differentiation experiments). ∗∗∗p < 0.001, Statistical significance was assessed by Wilcoxon test.