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. 2022 Apr 27;12(6):2624–2639. doi: 10.1016/j.apsb.2022.04.013

Figure 4.

Figure 4

Bioreducible LNPs and chalcogen-containing LNPs for the delivery of genome engineering proteins. (A) Schematic illustration of the disulfide-containing 8-O14B LNP-mediated GFP-Cre and CRISPR/Cas9 RNP delivery. (B) 8-O14B LNP-enabled Cre-mediated gene recombination in the brain tissue of Rosa26tdTomato mouse model. (C) Bioreducible LNPs can deliver Cas9/sgRNA RNP targeting the GFP gene and induce gene knockout in cultured GFP-HEK cell line. Reproduced with permission from Ref. 87. Copyright © 2016 National Academy of Sciences USA. (D) Chemical structures of chalcogen-containing lipids, R-O17O, R-O17S, and R-O17Se. (E) Chalcogen LNP-mediated GFP-Cre delivery to DsRed-HeLa cell line. Percentage of GFP positive cells shown for 51 LNPs tested. Data points marked in red for LNPs induced high level of transfection (>20%). (F) The tail structure (O17O, O17S and O17Se)-influenced GFP-Cre protein transfection activity. The relative hit rates for officious LNPs of lipids with O17O, O17S and O17Se tails are −11.9%, −4.7% and 16.7%, respectively, relative to the initial chalcogen-containing library. Reproduced with permission from Ref. 88. Copyright © 2018 Elsevier.