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. 2022 Feb 16;12(6):2658–2671. doi: 10.1016/j.apsb.2022.02.009

Figure 8.

Figure 8

Mechanisms of ARV-825 repressing M2 macrophages polarization. (A) The diagram of BRD4 acting on the IRF4 gene promoter. (B) The diagram of ARV-825 reducing luciferase gene transcription initiated by the IRF4 promoter through inhibiting BRD4. (C) Relative IRF4 luciferase reporter activity in RAW264.7 macrophages treated with or without ARV-825 for 48 h detected by dual-luciferase reporter assay (n = 3). (D) ChIP-qPCR analysis of IRF4 promoter in DMSO or ARV-825 treated RAW264.7 macrophages for 48 h using IgG or anti-BRD4 (n = 3). (E) Western blot analysis of proteins (P-STAT6, STAT6, P-AKT, AKT, P-STAT3 and STAT3) related to M2 macrophages polarization pathways. Data are presented as mean ± SEM. No significant difference is mark with ns. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 and ∗∗∗∗P < 0.0001.