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. 2022 Jun 8;12:759864. doi: 10.3389/fonc.2022.759864

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Copyright © 2022 Lin, Peng, Zhu, Xiong, Zhang and Lei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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STK25 overexpression inhibits the expression of tumor stem cell markers and EMT in HCC cells promoted by miR-4800-3p through the Hippo pathway. (A) The stemness of HepB3 and LM3 cells was evaluated using a sphere formation assay. qPCR and Western blot analysis of tumor stem cell markers (CD44, CD133, and OCT4) (B, C) and EMT biomarkers (E-cadherin, ZO-1, and N-cadherin) (D, E) in HepB3 and LM3 cells. (F) Western blot was used to detect the expression of p-YAP, YAP, TAZ, and PCNA in HepB3 and LM3 cells. *p < 0.05 and **p < 0.01 vs. mimic+OE-NC.