Fig. 2.

(A) Light microscopy images (a to g) and cytotoxicity assay (h) on vero-E6 cells at day 4 post-treatment with recovered 3% or 6% Tris-Glycine Beef buffer (TGB, pH 9.5) or with (B) recovered 3% Tris-Glycine beef buffer (TGB, pH 9.5) from berries. All samples were subjected to all the processing steps of FDA-based method and the pH was measured for all recovered eluants before being tested for their effect on cell viability at undiluted, 1:10 or 1:100 dilutions. Control negative cells were used to calculated % viability. Comparing within each buffer or berry type: means with different letters differ significantly (p < 0.05). Comparing between buffers or berry types: significant differences are denoted with asterisks.