TABLE 1.
Strains and plasmids used in this study.
| Strains or plasmids | Relevant properties | Source |
|---|---|---|
| E. coli DH5α | F−, φ80dlacZ△M1, △(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17 (rk−, mk+), phoA, supE44, λ− thi-1, gyrA96, relA1 | |
| E. coli GM2163 | F−, ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2 galT22 supE44 hisG4 rpsL 136 (Str R ) xyl-5 mtl-1 dam13::Tn9 (Cam R ) dcm-6 mcrB1 hsdR2 mcrA | Qiu et al. (2017) |
| B. amyloliquefaciens NB | Glutamate-independent B. amyloliquefaciens can produce high yield of polyglutamic acid |
Qiu et al. (2017) |
| B. amyloliquefaciens NB(ΔpgsBCA) | B. amyloliquefaciens NB derivate, deletion of pgsBCA | Sha et al. (2020a) |
| B. amyloliquefaciens NB(ΔpgsA) | B. amyloliquefaciens NB derivate, deletion of pgsA | This study |
| B. amyloliquefaciens NB(ΔpgsB) | B. amyloliquefaciens NB derivate, deletion of pgsB | This study |
| B. amyloliquefaciens NB(ΔpgsC) | B. amyloliquefaciens NB derivate, deletion of pgsC | This study |
| B. amyloliquefaciens NBO1 | NBΔpgsBCA derivate, deletion of argF | This study |
| B. amyloliquefaciens NBO2 | NBΔpgsBCA derivate, deletion of argI | This study |
| B. amyloliquefaciens NBO3 | NBO1 derivate, deletion of argI | This study |
| B. amyloliquefaciens NBO4 | NBO3 derivate, deletion of speF | This study |
| B. amyloliquefaciens NBO5 | NBO3 derivate, deletion of prob | This study |
| B. amyloliquefaciens NBO6 | NBO3 derivate, deletion of speF gene and prob gene | This study |
| B. amyloliquefaciens NBO7 | NBO6 derivate, carrying pHY-ArgA | This study |
| B. amyloliquefaciens NBO8 | NBO6 derivate, carrying pHY-ArgB | This study |
| B. amyloliquefaciens NBO9 | NBO6 derivate, carrying pHY-ArgC | This study |
| B. amyloliquefaciens NBO10 | NBO6 derivate, carrying pHY-ArgD | This study |
| B. amyloliquefaciens NBO11 | NBO6 derivate, carrying pHY-ArgE | This study |
| B. amyloliquefaciens NBO12 | NBO6 derivate, carrying pHY-ArgA-ArgE | This study |
| B. amyloliquefaciens NBO13 | NBO6 derivate, carrying pHY-ArgABCDE | This study |
| B. amyloliquefaciens NBO14 | NBO6 derivate, carrying pHY- Cg.lysE | This study |
| B. amyloliquefaciens NBO15 | NBO6 derivate, carrying pHY- E.coli.lysE | This study |
| B. amyloliquefaciens NBO16 | NBO6 derivate, carrying pHY- BS.lysE | This study |
| B. amyloliquefaciens NBO17 | NBO6 derivate, carrying pHY- BA.lysE | This study |
| B. amyloliquefaciens NBO18 | NBO12 derivate, overexpression of BA.lysE | This study |
| Plasmids | ||
| pHT-01 | Amp r , Cm r , E. coli−B. subtilis shuttle vector | Qiu et al. (2020a) |
| pDR244 | Amp r , Spec r , cre/lox-mediated E. coli-Bacillus shuttle vector | Qiu et al. (2020a) |
| pHY | Amp r , Cm r , E. coli−B. subtilis shuttle vector, containing the constitutive strong promoter P HpaII , p15A ori | Qiu et al. (2020a) |
| pHT-Cas9n | pHT derivate consists of the promoter P grac , Cas9n and the terminator T amy | Qiu et al. (2020a) |
| pDR-pgssgRNA | pDR derivate consists of the promoter P HpaⅡ , sgRNA, Tamy, the up and downstream fragment of pgsBCA gene | This study |
| pDR-argIsgRNA | pDR derivate consists of the promoter P HpaⅡ , sgRNA, Tamy, the up and downstream fragment of argI gene | This study |
| pDR-argFsgRNA | pDR derivate consists of the promoter P HpaⅡ , sgRNA, Tamy, the up and downstream fragment of argF gene | This study |
| pDR-speFsgRNA | pDR derivate consists of the promoter P HpaⅡ , sgRNA, Tamy, the up and downstream fragment of speF gene | This study |
| pDR-probsgRNA | pDR derivate consists of the promoter P HpaⅡ , sgRNA, Tamy, the up and downstream fragment of prob gene | This study |
| pHY-ArgA | pHY containing promoter P HpaⅡ , the gene ArgA and T amy terminator | This study |
| pHY-ArgB | pHY containing promoter P HpaⅡ , the gene ArgB and T amy terminator | This study |
| pHY-ArgC | pHY containing promoter P HpaⅡ , the gene ArgC and T amy terminator | This study |
| pHY-ArgD | pHY containing promoter P HpaⅡ , the gene ArgD and T amy terminator | This study |
| pHY-ArgE | pHY containing promoter P HpaⅡ , the gene ArgE and T amy terminator | This study |
| pHY-ArgA-ArgE | pHY containing promoter P HpaⅡ , the gene ArgA-ArgE and T amy terminator | This study |
| pHY-ArgABCDE | pHY containing promoter P HpaⅡ , the gene ArgA, ArgB, ArgC, ArgD, ArgE and T amy terminator | This study |
| pHY- Cg.lysE | pHY containing promoter P HpaⅡ , the gene Cg.lysE and T amy terminator | This study |
| pHY- E.coli.lysE | pHY containing promoter P HpaⅡ , the gene E.coli.lysE and T amy terminator | This study |
| pHY- BS.lysE | pHY containing promoter P HpaⅡ , the gene BS.lysE and T amy terminator | This study |
| pHY- BA.lysE | pHY containing promoter P HpaⅡ , the gene BA.lysE and T amy terminator | This study |
| pHY-ArgA-ArgE- BA.lysE | pHY containing promoter P HpaⅡ , the gene ArgA-ArgE- BA.lysE and T amy terminator | This study |