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. 2022 Jun 7;12:911294. doi: 10.3389/fonc.2022.911294

Figure 2.

Figure 2

HE, ALK IHC, ALK FISH, and NGS sequencing data of the five ALK FISH-positive cases. Panels (A, D, E, F) were diagnosed as LCNEC, whereas (B, C) were evaluated as (AC). IHC was scored as 2+ (A), 1+ (B), and 3+ (C–F). ALK FISH break-apart probes revealed aberrant staining patterns (A, D) one fused and one split red and green signal; (B, D, E) one fused and one single red signal) consistent with ALK rearrangements. In three cases (C–F), the material was sufficient to perform NGS, confirming two times an EML4-ALK translocation (D) Variant 3a/b; (E, F) Variant 1) and one time an STRN-ALK translocation (C). HE, hematoxylin–eosin; ALK, anaplastic lymphoma kinase; IHC, immunohistochemistry; FISH, fluorescence in situ hybridization; NGS, next-generation sequencing. Scale bar 200 µm.